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Related Concept Videos

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Stem Cell Niche01:26

Stem Cell Niche

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

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The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
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Related Experiment Video

Updated: Sep 27, 2025

Analysis of Hematopoietic Stem Progenitor Cell Metabolism
12:20

Analysis of Hematopoietic Stem Progenitor Cell Metabolism

Published on: November 9, 2019

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Hematopoietic stem cell metabolism and stemness.

Dan Huang1, Chiqi Chen1, Li Xie1

  • 1Hongqiao International Institute of Medicine, Shanghai Tongren Hospital, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Faculty of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Blood Science (Baltimore, Md.)
|April 11, 2022
PubMed
Summary

Hematopoietic stem cells (HSCs) rely on glycolysis and other nutrient metabolisms for their function and fate. Understanding these metabolic profiles is key to improving HSC therapies for blood disorders.

Keywords:
Bone marrow nicheGlycolysisHematopoietic stem cellsOxidative phosphorylationStemness

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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

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Related Experiment Videos

Last Updated: Sep 27, 2025

Analysis of Hematopoietic Stem Progenitor Cell Metabolism
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Analysis of Hematopoietic Stem Progenitor Cell Metabolism

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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
10:17

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Metabolic Regulation

Background:

  • Hematopoietic stem cells (HSCs) originate in the aorta-gonad-mesonephros and migrate to the bone marrow niche.
  • HSCs are crucial for treating hematopoietic disorders, but their limited availability hinders clinical application.
  • Understanding HSC stemness and cell fate determination during development is critical.

Purpose of the Study:

  • To review current knowledge on HSC metabolic profiles and regulatory networks.
  • To highlight the role of intrinsic metabolic regulation in HSC fate determination.
  • To identify key metabolic pathways and nutrient roles in HSC activity.

Main Methods:

  • Literature review of studies on HSC metabolism and regulation.
  • Analysis of intrinsic and extrinsic factors influencing HSC development.
  • Summary of identified regulatory pathways (e.g., MEIS1/HIF1A, MYC, PPM1K/CDC20, ROS).

Main Results:

  • HSCs primarily use glycolysis for energy but also require mitochondrial respiration.
  • Amino acid and lipid metabolism play unique roles in sustaining HSC activities.
  • Specific regulatory pathways fine-tune nutrient metabolism and cell fate commitment in HSCs.

Conclusions:

  • Intrinsic metabolic regulation is critical for HSC stemness and cell fate.
  • Further research is needed to develop sensitive metabolic techniques for HSC studies.
  • Exploring nutrient metabolism across HSC developmental stages is essential for future applications.