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Related Experiment Video

Updated: Sep 27, 2025

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System

Published on: December 5, 2020

Imaging Unique DNA Sequences in Individual Cells Using a CRISPR-Cas9-Based, Split Luciferase Biosensor.

Nicholas G Heath^1,2,3, Henriette O'Geen^1,3, Nicole B Halmai^1,3

¹Genome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, Davis, CA, United States.

Frontiers in Genome Editing

|April 11, 2022

Summary

This study introduces a novel CRISPR-based DNA biosensor using NanoLuc luciferase fragments. This "turn-on" probe offers sensitive detection of low-copy DNA sequences in live cells, outperforming traditional fluorescent probes.

Failed At:

2026-06-19T13:39:30.953741+00:00

Keywords:

CRISPR dCas9 live cell imaging single gene locus split reporter

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