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Updated: Sep 27, 2025

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
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Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

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Establishing spatial control over TORC1 signaling

Oliver Schmidt1, Mariana E G de Araujo1

  • 1Institute of Cell Biology, Biocenter Innsbruck, Medical University of Innsbruck, Innsbruck, Austria.

The Journal of Cell Biology
|April 11, 2022
PubMed

Insights

Target-of-rapamycin complex 1 (TORC1) is found on signaling endosomes. This study identifies the molecular components and mechanisms regulating these crucial endosomes.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Signal Transduction

Background:

  • Target-of-rapamycin complex 1 (TORC1) is a key regulator of cell growth and metabolism.
  • TORC1 localizes to lysosome-related organelles, including signaling endosomes.
  • The precise molecular composition and regulation of signaling endosomes remain incompletely understood.

Purpose of the Study:

  • To define the molecular identity of signaling endosomes.
  • To identify key proteins involved in the formation and maintenance of signaling endosomes.
  • To elucidate the role of TORC1 in signaling endosome biology.

Main Methods:

  • Utilized advanced microscopy techniques to visualize endosomal compartments.
  • Employed proteomic analysis to identify proteins associated with signaling endosomes.
  • Performed genetic manipulation to assess the function of identified proteins.

Main Results:

  • Characterized signaling endosomes as a distinct endosomal subpopulation.
  • Identified novel protein components essential for signaling endosome structure and function.
  • Demonstrated that TORC1 is a critical regulator of signaling endosome dynamics.

Conclusions:

  • Signaling endosomes possess a unique molecular signature.
  • Specific proteins are indispensable for the formation and maintenance of signaling endosomes.
  • TORC1 plays a vital role in regulating signaling endosome function, impacting cellular signaling pathways.

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