Synergistic promoting effect of increasing aquatic ammonium and CO₂ on Microcystis aeruginosa

Owing to climate change and intensive agricultural development, freshwater bodies have been affected by increases in both CO₂ levels and chemically-reduced forms of N. However, little is known about how these changes affect cyanobacterial growth and blooms. This study explored a range of light conditions (30, 80, 130, or 200 μmol photons/m²/s) wherein Microcystis aeruginosa, a widespread bloom-forming species, was exposed to different concentrations of CO₂ (400 parts per million (ppm) and 1000 ppm) in a medium containing NH₄⁺ or NO₃－. The interactive effects of N sources and CO₂ levels on the C/N metabolic balance and energy balance were examined to assess changes in the growth of M. aeruginosa. When the light intensity was 80 μmol photons/m²/s, elevated CO₂ could reduce intracellular reactive oxygen species (ROS) in NH₄⁺-grown M. aeruginosa. Meanwhile, cell density and chlorophyll a (Chl a) increased with increasing CO₂ levels, and the increase in Chl a was significantly greater in NH₄⁺-grown M. aeruginosa than in NO₃－-grown M. aeruginosa. Under light conditions of 200 μmol photons/m²/s, elevated CO₂ concentration caused NO₃－-grown M. aeruginosa to be affected by a large amount of ROS, and the growth of NO₃－-grown M. aeruginosa was finally suppressed. However, NH₄⁺-grown M. aeruginosa had a smaller amount of ROS and showed improved growth as CO₂ was elevated. This difference can be attributed to the faster metabolic pathways in the NH₄⁺ environment, which manifested in a lower accumulation of 2-oxoglutarate and fatty acids as CO₂ was elevated. These findings suggest that the simultaneous increase in ammonium and CO₂ in aquatic ecosystems confers cyanobacteria with greater advantages than the combination of nitrate and CO₂, which may aggravate cyanobacterial blooms.