Identification of key water parameters and microbiological compositions triggering intensive N₂O emissions during...

Landfill leachate treatment processes tend to emit more N₂O compared to domestic wastewater treatment. This discrepancy may be ascribed to leachate water characteristics such as high refractory COD, ammonium (NH₄⁺) content, and salinity. In this work, the leachate influent was varied to examine the N₂O emission scenarios. NH₄⁺-N, COD, and Cl^- concentrations ranged between 1000-2500, 1000-10,000, and 500-3000 mg L^-1, respectively. Simultaneously, we attempted to combine statistical analysis with high-throughput sequencing to understand the microbial mechanism with regards to N₂O emission. Results show that the strong N₂O emissions occur in the nitrifying tank due to the intensive aeration. The system receiving the lowest COD shows the maximum N₂O emission factor of 42.7% of the removed nitrogen. Both redundancy analysis and a structural equation model verify that insufficient degradable organics are the key water parameter triggering intensive N₂O emission within the designed influent limits. Furthermore, two essential but non-abundant functional bacteria, Flavobacterium (acting as a denitrifier) and Nitrosomonas (acting as a nitrifier), are identified as the core functional species that dramatically influence N₂O emissions. An increase in influent COD promotes the proliferation of Flavobacterium and inhibits Nitrosomonas, which in turn reduce N₂O release. Meanwhile, two keystone species of Castellaniella and Saprospiraceae unclassified are recognized. They may supply a suitable niche and integrity of the microbial community for N-cycle functional bacteria. These findings reveal the essential role of non-abundant species in microbial community, and expand the current understanding of microbial interactions underlying N₂O dynamics in leachate treatment systems.