Related Experiment Video
Updated: Sep 27, 2025

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
Published on: April 24, 2018
Can Lemna minor mitigate the effects of cadmium and nickel exposure in a Neotropical fish?
Vanessa Bezerra1, Wagner Ezequiel Risso1, Claudia Bueno Dos Reis Martinez1
1Department of Physiological Sciences, Londrina State University, Rodovia Celso Garcia Cid, Km 380, s/n - Campus Universitário, Londrina, PR CP 86057-970, Brazil.
Abstract:
We aimed to evaluate if Lemna minor can mitigate the observed effects of cadmium (Cd) and nickel (Ni) exposure in Prochilodus lineatus. Fish were exposed for 96 h to 20 µg L-1 of Cd, 1.5 mg L-1 of Ni, or to a mixture of these two metals. In all tests, one group was exposed to the metals with duckweed on the water surface, and other group was exposed only to the metals, without plants. After each exposure, samples of P. lineatus tissues were collected to evaluate multiple biomarkers. Duckweed prevented bioaccumulation in some fish tissues and attenuated changes in acetylcholinesterase activity, increases in erythrocytic nuclear abnormality frequency, and hyperglycemia. However, the changes in plasma ion concentrations, reduction in activity of ion transport enzymes, and histological damage were not mitigated. Therefore, we concluded that L. minor partially attenuates the effects caused by Cd and Ni exposure.

