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Updated: Sep 27, 2025
Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Highly catalysis MOFCe supported Ag nanoclusters coupled with specific aptamer for SERS quantitative assay of trace
Jinling Shi1, Jingjing Li1, Aihui Liang1
1Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China.
Abstract:
A cerium metal organic framework-loaded silver nanocluster (MOFCeAgNC) is synthesized by a facile stirring procedure with trimesic acid, cerium nitrate, silver nitrate and NaBH4, which exhibites strong catalytic activity in the indicated reaction of HAuCl4-sodium lactate (SL). MOFCeAgNC can sensitively detect dopamine (DA) by surface-enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) techniques with a detection concentration range of 0.01-0.25 nmol/L and a detection limit of 0.008 nmol/L. Based on the specific binding of aptamer (Apt)-DA and the catalytic amplification strategy of MOFCeAgNC on HAuCl4-SL, a sensitive and convenient DA platform for dual-mode detection of SERS and RRS is constructed. In addition, the platform is successfully applied to detect DA in human serum with satisfactory recoveries (95.7-102%).
