Minimum Alveolar Concentration of Isoflurane in Rats Chronically Treated with the Synthetic Cannabinoid WIN 55,212-2
Julio Raul Chavez-Monteagudo1, José Antonio Ibancovichi2, Pedro Sanchez-Aparicio3
1Department of Veterinary Anesthesia and Analgesia, Facultad de Estudios Superiores Cuautitlán, Hospital de Pequeñas Especies, Universidad Nacional Autónoma de México, Cuautitlan Izcalli 54740, Mexico.
Abstract:
The minimum alveolar concentration MAC of isoflurane was measured in rats chronically treated with WIN 55,212-2.
Methods:
The MAC of isoflurane was determined in 24 male rats from expiratory samples at time of tail clamping under the following conditions: without treatment MAC(ISO), in rats treated for 21 days with WIN 55,212-2 MAC(ISO + WIN55), and in rats 8 days after stopping treatment with WIN 55,212-2 (MACISO + WIN55 + 8D).
Results:
The MAC(ISO) was 1.32 ± 0.06. In the MAC(ISO + WIN55) group, the MAC increased to 1.69 ± 0.09 (28%, p-value ≤ (0.0001). Eight days after stopping treatment with WIN55, the MAC did not decrease significantly, 1.67 ± 0.07 (26%, p-value ≤ 0.0001).
Conclusions:
The administration of WIN 55,212-2 for 21 days increases the MAC of isoflurane in rats. This effect does not disappear 8 days after discontinuation of treatment with the synthetic cannabinoid.
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