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Related Experiment Videos

Genetically essential and nonessential alpha-tubulin genes specify functionally interchangeable proteins.

P J Schatz, F Solomon, D Botstein

    Molecular and Cellular Biology
    |November 1, 1986
    PubMed
    Summary

    The budding yeast Saccharomyces cerevisiae has two alpha-tubulin genes, TUB1 and TUB3. While TUB1 is essential for growth, TUB3 is not, though both genes perform similar functions in cell division and mating.

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    Area of Science:

    • Cell Biology
    • Genetics
    • Molecular Biology

    Background:

    • Microtubules are crucial for cell division and nuclear movement in yeast.
    • Budding yeast Saccharomyces cerevisiae possesses two distinct alpha-tubulin genes: TUB1 and TUB3.

    Purpose of the Study:

    • To investigate the relative importance of TUB1 and TUB3 in yeast mitosis, meiosis, and mating.
    • To understand the functional relationship between the two alpha-tubulin genes.

    Main Methods:

    • Construction and analysis of null mutations for TUB1 and TUB3.
    • Gene copy number alterations using chromosomal and plasmid-based approaches.

    Main Results:

    • TUB3 null mutations were not essential for mitosis, meiosis, or mating but caused benomyl hypersensitivity and reduced spore viability.

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  • TUB1 null mutations were essential for haploid cell growth, with heterozygous diploids showing slow growth and poor sporulation.
  • Overexpression of either TUB1 or TUB3 could suppress the defects caused by a null mutation in the other gene.

    • Conclusions:

    • Despite a 10% divergence, TUB1 and TUB3 exhibit no essential qualitative functional differences in yeast.
    • Differential expression levels likely account for the observed functional distinctions between TUB1 and TUB3.