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Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

2.8K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.8K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.6K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.6K

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Related Experiment Video

Updated: Sep 27, 2025

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells
09:54

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Published on: April 18, 2019

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Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In

Sophie Domingues1, Annabelle Darle1, Yolande Masson1

  • 1Centre d'Etude des Cellules Souches, 91100 Corbeil-Essonnes, France.

Cells
|April 12, 2022
PubMed
Summary

Novel stem cell-derived skin substitutes offer a promising solution for chronic wounds, like sickle cell leg ulcers. These engineered tissues provide a potential new therapeutic avenue for difficult-to-heal skin conditions.

Keywords:
GMP compliantfibroblastskeratinocytespluripotent stem cellsskin tissue engineeringwound healing

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Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

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Related Experiment Videos

Last Updated: Sep 27, 2025

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Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

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Area of Science:

  • Regenerative Medicine
  • Dermatology
  • Stem Cell Biology

Background:

  • Chronic wounds, including sickle cell disease leg ulcers, present a significant clinical challenge due to prolonged inflammation and limited effective treatments.
  • Current cell- and tissue-based therapies are restricted by the limited sourcing of primary cells for manufacturing.
  • There is a critical need for advanced wound healing solutions and novel skin substitutes.

Purpose of the Study:

  • To develop Good Manufacturing Practices compliant (GMPc) protocols for producing functional keratinocytes and fibroblasts from pluripotent stem cells.
  • To engineer a dermo-epidermal skin substitute using these stem cell-derived cells and a plasma-based fibrin matrix.
  • To evaluate the biological activity and wound healing potential of the manufactured composite skin substitute.

Main Methods:

  • Utilized pluripotent stem cells to generate keratinocytes and fibroblasts under GMPc conditions.
  • Reconstructed a composite skin substitute by integrating these cells with a plasma-based fibrin matrix.
  • Assessed the in vitro biological activity, including enhancement of keratinocyte wounding.

Main Results:

  • Successfully established GMPc protocols for stem cell-derived keratinocyte and fibroblast production.
  • Manufactured a biologically active composite skin substitute.
  • Demonstrated that the engineered skin enhances in vitro keratinocyte wound healing.

Conclusions:

  • The proposed method enables the production of functional dermo-epidermal skin substitutes from pluripotent stem cells.
  • This engineered skin substitute shows promise for treating chronic wounds and offers new perspectives for allogeneic skin replacement therapies.