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Effectiveness of Direct Laser Interference Patterning and Peptide Immobilization on Endothelial Cell Migration for
Romain Schieber1,2,3, Carlos Mas-Moruno1,2, Federico Lasserre3
1Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Barcelona East School of Engineering (EEBE), Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany, 10-14, 08019 Barcelona, Spain.
Nanomaterials (Basel, Switzerland)
|April 12, 2022
Summary
This study enhanced cardiovascular stent surfaces with topographical patterns and peptides, significantly improving endothelial cell adhesion, migration, and proliferation for better stent endothelialization.
Area of Science:
- Biomaterials Science
- Surface Engineering
- Cell Biology
Background:
- Cardiovascular stent implantation can lead to restenosis and late-stent thrombosis due to incomplete endothelial coverage.
- Modifying stent surfaces with topographical or biochemical cues can promote endothelial cell (EC) functions.
Purpose of the Study:
- To investigate the synergistic effects of combining topographical linear patterning and peptide functionalization on cobalt-chromium (CoCr) alloy surfaces.
- To enhance endothelial cell adhesion, migration, and proliferation on modified CoCr stent surfaces.
Main Methods:
- Linear patterns were created on CoCr surfaces using direct laser interference patterning (DLIP) with varying depths.
- Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides were immobilized onto the patterned surfaces.
- Surface characterization (AFM, XPS) and biological assays (EC adhesion, migration, proliferation) were performed.
Main Results:
- DLIP successfully created 10 µm periodic patterns with depths of ≈79 and 762 nm.
- Peptide functionalization, particularly with YIGSR, increased early EC adhesion.
- High-depth patterns promoted EC alignment and migration, with synergistic acceleration of EC migration and proliferation observed when combining topography and peptides.
Conclusions:
- Combined topographical patterning and peptide functionalization synergistically enhance EC response on CoCr surfaces.
- This strategy holds significant potential for improving cardiovascular stent endothelialization and reducing adverse clinical events.
