Leaf Photosynthesis and Its Temperature Response Are Different between Growth Stages and N Supplies in Rice Plants

Leaf photosynthesis is highly correlated with CO₂-diffusion capacities, which are determined by both leaf anatomical traits and environmental stimuli. In the present study, leaf photosynthetic rate (A), stomatal conductance (g_s), mesophyll conductance (g_m) and the related leaf anatomical traits were studied on rice plants at two growth stages and with two different N supplies, and the response of photosynthesis to temperature (T) was also studied. We found that g_m was significantly higher at mid-tillering stage and at high N treatment. The larger g_m was related to a larger chloroplast surface area facing intercellular air spaces and a thinner cell wall in comparison with booting stage and zero N treatment. At mid-tillering stage and at high N treatment, g_m showed a stronger temperature response. The modelling of the g_m-T relationships suggested that, in comparison with booting stage and zero N treatment, the stronger temperature response of g_m was related to the higher activation energy of the membrane at mid-tillering stage and at high N treatment. The findings in the present study can enhance our knowledge on the physiological and environmental determinants of photosynthesis.