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Published on: January 12, 2020
PTH2R is related to cell proliferation and migration in ovarian cancer: a multi-omics analysis of bioinformatics and
Wang Xiaowei1, Lu Tong2, Qu Yanjun1
1Department of Ultrasnography in Gynecology and Obstetrics, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Background:
Ovarian cancer is a common gynecological disease and seriously endangers women's health. Currently, there is still a lack of effective molecular markers for the diagnosis and treatment of ovarian cancer. The present study aimed to investigate the molecular markers associated with ovarian cancer.
Methods:
The molecular and gene related to ovarian cancer were extracted from GEO database and TCGA database by bioinformatics, and the related genes and functions were further analyzed. The results were verified by qPCR, WB, CCK-8 and Transwell experiments.
Results:
Data analysis showed that PTH2R gene was highly expressed in tumors, and 51 HUB genes were obtained. Finally, experimental verification showed that PTH2R gene was highly expressed in ovarian cancer, and PTH2R gene was involved in the proliferation, invasion and metastasis of ovarian cancer cells.
Conclusions:
After experimental verification, we found that knocking down the expression of PTH2R can inhibit the proliferation, invasion and migration of tumor cells.PTH2R is expected to become a new molecular marker for ovarian cancer.
Insights
Parathyroid hormone receptor 2 (PTH2R) is highly expressed in ovarian cancer, promoting tumor cell proliferation, invasion, and metastasis. Inhibiting PTH2R shows potential for new ovarian cancer treatments and diagnostic markers.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Ovarian cancer poses a significant threat to women's health.
- Effective molecular markers for ovarian cancer diagnosis and treatment are lacking.
Purpose of the Study:
- To identify novel molecular markers for ovarian cancer.
- To investigate the role of specific genes in ovarian cancer development.
Main Methods:
- Bioinformatic analysis of GEO and TCGA databases to identify key genes.
- Experimental validation using qPCR, Western Blot (WB), CCK-8, and Transwell assays.
Main Results:
- The PTH2R gene was found to be highly expressed in ovarian tumors.
- PTH2R gene expression correlated with increased proliferation, invasion, and metastasis of ovarian cancer cells.
Conclusions:
- Downregulating PTH2R expression inhibits ovarian tumor cell proliferation, invasion, and migration.
- PTH2R presents potential as a novel molecular marker for ovarian cancer.
