Exopolysaccharides production from marine Bacillus strains and their antioxidant and bio-flocculant capacities

Microbial exopolysaccharides (EPS) have gained high scientific concern due to their exceptional physicochemical features and high industrial applicability. Owing to their biotechnological importance, the present study was designed to screen and isolate the EPS-producing Bacillus strains based on their growth potential on specific media and colony morphologies. The bacterial isolates Bacillus subtilis Bs1-01, Bacillus licheniformis Bl1-02, and Bacillus brevis Bb1-04 showed excellent EPS production due to their shortened lag phase and abundant biomass production. Shake-flask fermentation valued the maximum production yield of 50.19 ± 1.14 g/L by Bl1-02 after 72 h incubation (about 3.40 times higher than that of Bacillus thuringiensis Bt1-05). The basic component analysis revealed the improved amount of total carbohydrate, reducing sugar ends, and protein contents by Bl1-02 strain. Structural characteristics and functional groups of the EPS characterized by Fourier transform infrared spectroscopy demonstrated that all EPS were in close agreement to each other due to the presence of similar chemical bonds and functional groups. EPS from Bl1-02 strain showed stronger and more stable bio-emulsifying and hygroscopicity activities (12.23%). The crude EPS exhibited potent antioxidant properties which were examined against reducing potential (H₂O₂ scavenging) and total antioxidant tests. Among bio-flocculation activities of EPS at different concentrations, Bs1-01 strain produced EPS at a concentration of 60 mg/mL was observed to show the maximum value of 79.20%. In conclusion, the EPS from marine Bacillus strains showed excellent functional properties suggesting potential industrial applications that demand separate investigations.