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Updated: Sep 27, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

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Long non-coding RNA Opa interacting protein 5-antisense RNA 1 binds to micorRNA-34a to upregulate oncogenic PD-L1 in

Xinwei Qiao1, Feng Zhao1

  • 1Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province, China.

Bioengineered
|April 12, 2022
PubMed

Insights

Long non-coding RNA OIP5-AS1 is upregulated in non-small-cell lung cancer (NSCLC), promoting cancer cell proliferation by increasing PD-L1 via sponging miR-34a.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNA (lncRNA) OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) has an oncogenic role in various cancers.
  • The involvement of OIP5-AS1 in non-small-cell lung cancer (NSCLC) remains largely uncharacterized.
  • Preliminary data indicate OIP5-AS1 is upregulated in NSCLC tissues.

Purpose of the Study:

  • To investigate the role of OIP5-AS1 in NSCLC.
  • To elucidate the molecular mechanism by which OIP5-AS1 influences NSCLC progression.
  • To explore the relationship between OIP5-AS1, miR-34a, and programmed death-ligand 1 (PD-L1) in NSCLC.

Main Methods:

  • Gene expression analysis using RT-qPCR.
  • RNA-RNA pull-down assays to confirm direct RNA interactions.
  • Cell proliferation assays (CCK-8, colony formation).
  • Overexpression and inhibition studies of OIP5-AS1 and miR-34a.

Main Results:

  • OIP5-AS1 expression is significantly upregulated in NSCLC.
  • OIP5-AS1 overexpression increases PD-L1 levels and promotes NSCLC cell proliferation.
  • OIP5-AS1 acts as a sponge for miR-34a, inhibiting its function.
  • miR-34a inhibition or OIP5-AS1 silencing reverses the pro-proliferative effects and PD-L1 upregulation.

Conclusions:

  • OIP5-AS1 promotes NSCLC cell proliferation by upregulating oncogenic PD-L1 through sponging miR-34a.
  • OIP5-AS1 and miR-34a are dysregulated in NSCLC, suggesting their potential as therapeutic targets.
Keywords:
OIP5-AS1PD-L1miR-34anon-small cell lung cancerproliferation

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