An undergraduate laboratory to detect viruses in human DNA samples using qPCR

The COVID-19 pandemic has necessitated the need to reliably detect the presence of viral genomes in human clinical samples. The most accurate viral tests involve the use of qPCR. Thus, it is important for students to understand the mechanism to detect viral genomes by qPCR including critical qPCR controls and how to properly interpret qPCR data. Herein, we describe a 2-week undergraduate laboratory to detect a viral genome in a human DNA sample. The strategy follows a SARS-CoV-2 qPCR test in numerous ways. Students are provided isolated DNA representing a mock human patient sample, and determine if a viral genome (bacteriophage lambda) is present using qPCR. A battery of control samples and patient pooling strategies are utilized. The laboratory exercise is successful based on high rates of student success in detecting viral genomes, pre-quiz and post-quiz assessments focusing on viral qPCR testing, and positive student comments.