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Related Concept Videos

Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Riboswitches01:56

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
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Related Experiment Video

Updated: Sep 27, 2025

mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

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Localizing Total mRNA in Plant Cells.

Geraint Parry1

  • 1GARNet, School of Biosciences, Cardiff University, Cardiff, UK. arabidopsiseventsuk@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|April 12, 2022
PubMed
Summary

Visualizing cellular mRNA location is key to understanding gene function. In situ hybridization helps quantify mRNA accumulation in plant cell nuclei, aiding RNA processing and transport studies.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Plant Science

Background:

  • Understanding gene function requires knowledge of mRNA localization within cells.
  • The plant cell nucleus is a critical site for RNA processing and transport.
  • The nuclear pore complex plays a vital role in regulating molecular traffic between the nucleus and cytoplasm.

Purpose of the Study:

  • To highlight the importance of visualizing the total cellular mRNA pool.
  • To review the application of in situ hybridization for studying nuclear mRNA.
  • To connect mRNA localization with cellular physiology and gene expression.

Main Methods:

  • In situ hybridization protocols have been developed and refined over the past decade.
  • These methods allow for the visualization of mRNA distribution within the plant cell.
Keywords:
In situ hybridizationNuclear pore complexNuclear transportNucleusmRNA

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  • Quantification of mRNA accumulation in specific cellular compartments, particularly the nucleus, is enabled.

    • Main Results:

    • In situ hybridization enables precise localization of the total cellular mRNA pool.
    • Researchers have successfully visualized and quantified nuclear mRNA accumulation.
    • This technique supports investigations into RNA processing and nuclear transport mechanisms.

    Conclusions:

    • Visualizing mRNA location provides critical insights into gene function and cellular physiology.
    • In situ hybridization is a powerful tool for studying nuclear mRNA dynamics in plants.
    • Further research in RNA processing and nuclear transport benefits from these visualization techniques.