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Single-Molecule Imaging of Nuclear Transport
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Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter

Alexander Lee1,2, Marie A Bogoyevitch2, David A Jans3,4

  • 1Nuclear Signalling Laboratory, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|April 12, 2022
PubMed

Bimolecular fluorescence complementation utilizes the ability of two complementary nonfluorescent fragments to reconstitute and emit fluorescence when brought together through specific interaction of attached protein fragments of interest. It has been used in several different contexts to study protein-protein interaction. Here we apply the method for the first time to study interaction of the nuclear transporter importin α and its cargoes in a cellular context. By using image analysis to quantify the extent of nuclear complexation, it is possible to gain insight into the strength of interaction in cells.

Keywords:
Bimolecular fluorescence complementationCLSMImportin αNuclear transport

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