Related Experiment Video

Updated: Sep 27, 2025

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate
07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

3.8K

Analysis of the function and mechanism of DIRAS1 in osteosarcoma

Huan Liu1, Weibin Shu2, Tianyue Liu1

  • 1Department of Osteology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, China.

Tissue & Cell
|April 12, 2022
PubMed
Abstract

Insights

DIRAS1, regulated by METTL3/METTL14, impacts osteosarcoma cell behavior by affecting the ERK pathway. This study reveals DIRAS1

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a common bone cancer known for lung metastasis.
  • The role of DIRAS family GTPase 1 (DIRAS1) in osteosarcoma remains unclear.

Purpose of the Study:

  • To investigate the function and mechanism of DIRAS1 in osteosarcoma.
  • To explore the regulatory relationship between DIRAS1, METTL3/METTL14, and the ERK pathway in osteosarcoma.

Main Methods:

  • Western blot and immunofluorescence were used to analyze DIRAS1 expression and localization.
  • Cell proliferation, invasion, migration, and apoptosis were assessed using various assays.
  • RNA interference and plasmid transfection modulated DIRAS1 expression.
  • Methylation levels (m6A) and protein expression (p-ERK, p-AKT) were quantified.
  • Rescue experiments evaluated the interplay between METTL3/METTL14 and DIRAS1.

Main Results:

  • DIRAS1 is localized in the nucleus of osteosarcoma cells.
  • DIRAS1 silencing enhanced proliferation, invasion, and migration while inhibiting apoptosis.
  • DIRAS1 regulated p-ERK expression but not p-AKT.
  • METTL3/METTL14 suppressed DIRAS1 expression.
  • METTL3/METTL14 reversed the effects of DIRAS1 overexpression on cell behavior and p-ERK levels.

Conclusions:

  • DIRAS1, modulated by METTL3 and METTL14, significantly influences osteosarcoma cell malignancy.
  • The ERK pathway is a key mediator in DIRAS1's effect on osteosarcoma progression.
Keywords:
DIRAS1METTL14METTL3MigrationOsteosarcomaProliferation

More Related Videos

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma08:07

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

7.3K
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.0K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.1K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.4K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.3K