Related Experiment Video
Updated: Sep 27, 2025

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Improving myofibrillar proteins solubility and thermostability in low-ionic strength solution: A review
Ke Wang1, Yan Li2, Yimin Zhang3
1College of Food Science & Engineering, Qingdao Agricultural University, Qingdao 266109, China; College of Food Science & Engineering, Shandong Agricultural University, Tai'an 271018, China.
Developing myofibrillar protein drinks (MPDs) is challenging due to insoluble myofibrillar proteins (MPs) in low ionic strength solutions and heat-induced aggregation. This review explores strategies to improve MP solubility and thermostability for better MPD development.
Area of Science:
- Food Science and Technology
- Protein Chemistry
- Biochemistry
Background:
- Myofibrillar proteins (MPs) are a valuable source of meat protein nutrition.
- Developing myofibrillar protein drinks (MPDs) is hindered by the poor solubility and heat-induced aggregation of MPs in low ionic strength solutions.
- Current methods to improve MP solubility often involve inhibiting myofilament assembly.
Purpose of the Study:
- To review chemical and physical strategies for enhancing the solubility and thermostability of myofibrillar proteins (MPs).
- To provide insights into the mechanisms of myosin filament assembly and cross-linking aggregation.
- To offer novel ideas for the development of myofibrillar protein drinks (MPDs).
Main Methods:
- Review of existing literature on chemical and physical modification strategies for MPs.
- Analysis of myosin filament assembly processes.
- Examination of cross-linking aggregation mechanisms.
Main Results:
- Identified challenges in MP solubility and thermostability for MPD formulation.
- Detailed advantages, disadvantages, and recent advancements in various treatment strategies.
- Elucidated the role of myosin filament assembly and aggregation in protein stability.
Conclusions:
- Understanding MP solubility and thermostability is crucial for MPD development.
- Chemical and physical strategies offer potential solutions to improve MP functionality.
- Further research into these strategies can lead to innovative MPD products.

