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SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex.

Viviana Vásquez¹, Maria-Cristina Navas², Javier A Jaimes³

¹Max Planck Tandem Group in Nanobioengieneering, Institute of Chemistry, Faculty of Natural and Exact Sciences, University of Antioquia, Complejo Ruta N, Calle 67 Nº 52-20, Medellín, 050010, Colombia.

Analytica Chimica Acta

|April 13, 2022

Summary

A novel immunosensor rapidly detects SARS-CoV-2 spike protein for COVID-19 diagnosis. This device uses magnetic beads and antibodies, offering a sensitive and reproducible method for detecting the virus in patient samples.

Keywords:

ACE2 Coronavirus Immunosensor Magnetic beads SARS-CoV-2 Spike protein

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Area of Science:

Biomedical Engineering
Immunosensors
Viral Diagnostics

Background:

Accurate and rapid diagnosis of COVID-19 is crucial for pandemic control.
The SARS-CoV-2 spike protein is a key target for viral detection.

Purpose of the Study:

To develop and validate a novel immunosensor for the rapid detection of SARS-CoV-2 spike protein.
To assess the sensitivity, reproducibility, and clinical applicability of the developed immunosensor.

Main Methods:

Fabrication of an immunosensor using magnetic beads, anti-spike and anti-ACE2 antibodies, and HRP-labeled streptavidin.
Detection of spike protein via sandwich assay on screen-printed gold electrodes.
Electrochemical signal measurement using a portable potentiostat.

Main Results:

The immunosensor demonstrated high sensitivity with a limit of detection of 22.5 ng/mL.
High reproducibility was observed in the detection of spike protein.
The device successfully detected SARS-CoV-2 spike protein in patient nasopharyngeal swab samples.

Conclusions:

The developed immunosensor provides a rapid and sensitive method for SARS-CoV-2 detection.
This technology has the potential for point-of-care diagnostics, bringing detection closer to the patient.
The immunosensor shows promise for effective COVID-19 screening and management.