Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variation in the temporal expression of overlapping baculovirus transcripts.

T H Mainprize, K Lee, L K Miller

    Virus Research
    |October 1, 1986
    PubMed
    Summary

    This study examined gene expression in Autographa californica nuclear polyhydrolysis virus (AcNPV) by analyzing complementary DNA (cDNA) transcribed at different infection times. Results reveal overlapping RNA sets with unique temporal regulation and abundance, indicating complex viral gene expression patterns.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Analysis of two transcription activation elements in the promoter of the developmentally regulated con-10 gene of Neurospora crassa.

    Fungal genetics and biology : FG & B·1998
    Same author

    Tissue-specific repression of starvation and stress responses of the Neurospora crassa con-10 gene is mediated by RCO1.

    Fungal genetics and biology : FG & B·1998
    Same author

    Structural modification of an orally active thrombin inhibitor, LB30057: replacement of the D-pocket-binding naphthyl moiety.

    Bioorganic & medicinal chemistry·1998
    Same author

    The still-face effect in Chinese and Canadian 3- to 6-month-old infants.

    Developmental psychology·1998
    Same author

    Effects of intravenous immunoglobulin on plasma interleukin-10 levels in Kawasaki disease.

    Immunology letters·1998
    Same author

    Randomized trial of pamidronate in patients with thyroid cancer: bone density is not reduced by suppressive doses of thyroxine, but is increased by cyclic intravenous pamidronate.

    The Journal of clinical endocrinology and metabolism·1998

    Area of Science:

    • Molecular Biology
    • Virology
    • Genomics

    Background:

    • Autographa californica nuclear polyhydrolysis virus (AcNPV) is a widely used baculovirus expression vector.
    • Understanding AcNPV gene expression is crucial for optimizing its use in biotechnology and research.

    Purpose of the Study:

    • To investigate the temporal gene expression patterns of AcNPV.
    • To characterize the complementary DNA (cDNA) transcribed from AcNPV RNA at early and late infection stages.

    Main Methods:

    • Synthesis of cDNA from polyadenylated RNA at 2 and 10 hours postinfection (p.i.).
    • Cloning of cDNA into Escherichia coli using plasmid pUC-9.
    • Northern blot hybridization using cDNA probes to analyze RNA transcripts.

    Related Experiment Videos

    Main Results:

    • 18 cDNA plasmids from 2 h p.i. mapped to 5 viral genome regions.
    • 49 cDNA plasmids from 10 h p.i. mapped to 15 regions, including the 5 from 2 h p.i.
    • Northern blot analysis revealed overlapping sets of RNAs across different genome regions, with some showing sequential appearance of higher molecular weight RNAs.

    Conclusions:

    • AcNPV gene expression involves complex, overlapping RNA transcripts.
    • Temporal regulation and abundance vary significantly among these transcripts.
    • The findings provide insights into the dynamic nature of AcNPV RNA production during infection.