Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

293
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
293
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

2.7K
A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
2.7K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

1.6K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
1.6K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

5.1K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
5.1K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

560
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
560
IR Spectrometers01:25

IR Spectrometers

1.5K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coefficient-metric gradient-based digital wavefront correction for full-field swept-source optical coherence tomography.

Biomedical optics express·2026
Same author

Quantitative assessment of chlorine gas inhalation injury based on endoscopic OCT and spectral encoded interferometric microscope imaging with deep learning.

APL photonics·2024
Same author

OCT angiography in the monitoring of vaginal health.

APL bioengineering·2023
Same author

Polarization-Diversity Optical Coherence Tomography Assessment of Choroidal Nevi.

Investigative ophthalmology & visual science·2023
Same author

Optical coherence tomography evaluation of vaginal epithelial thickness during CO<sub>2</sub> laser treatment: A pilot study.

Journal of biophotonics·2022
Same author

Graph-based rotational nonuniformity correction for localized compliance measurement in the human nasopharynx.

Biomedical optics express·2021
Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026
Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026
Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026
Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026
Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026
Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026
See all related articles

Related Experiment Video

Updated: Sep 27, 2025

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
10:22

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements

Published on: September 7, 2019

8.4K

Numerical calibration method for a multiple spectrometer-based OCT system.

Yusi Miao1, Jun Song2, Destiny Hsu3

  • 1Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC, Canada.

Biomedical Optics Express
|April 13, 2022
PubMed
Summary
This summary is machine-generated.

This study presents a numerical spectral calibration method for multiple spectrometer-based spectral-domain optical coherence tomography (SD-OCT) systems. This approach simplifies alignment, improving in vivo retinal imaging without artifacts.

More Related Videos

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
06:50

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Published on: November 8, 2019

6.7K
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.4K

Related Experiment Videos

Last Updated: Sep 27, 2025

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
10:22

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements

Published on: September 7, 2019

8.4K
O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
06:50

O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

Published on: November 8, 2019

6.7K
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.4K

Area of Science:

  • Biomedical Optics
  • Medical Imaging Technology
  • Ophthalmic Imaging

Background:

  • Multiple spectrometer systems in spectral-domain optical coherence tomography (SD-OCT) require precise alignment.
  • Traditional hardware-based alignment is complex and time-consuming.
  • Misalignment leads to artifacts and degraded image quality in SD-OCT.

Purpose of the Study:

  • To introduce a novel numerical spectral calibration method for multi-spectrometer SD-OCT systems.
  • To provide an easy and practical alternative to hardware-based spectrometer alignment.
  • To demonstrate the effectiveness of numerical calibration for artifact-free retinal imaging.

Main Methods:

  • A numerical spectral calibration algorithm was developed and applied.
  • Interferometric signatures of a mirror sample in spatial and frequency domains were used.
  • Pixels corresponding to the same wavelength across spectrometers were identified numerically.
  • The method was tested on a dual-spectrometer SD-OCT system for in vivo retinal imaging.

Main Results:

  • The numerical calibration method successfully aligned multiple spectrometers.
  • Artifact-free in vivo retinal images were acquired using the calibrated dual-spectrometer SD-OCT.
  • High-speed and dual-balanced acquisition modes demonstrated the utility of the method.
  • Spectral alignment was confirmed as critical for high-quality SD-OCT imaging.

Conclusions:

  • Numerical spectral calibration offers a practical and efficient solution for multi-spectrometer SD-OCT systems.
  • This method enhances image quality by eliminating artifacts caused by spectrometer misalignment.
  • The technique is crucial for advancing high-performance ophthalmic imaging applications.