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Derivatizing Nile Red fluorophores to quantify the heterogeneous polarity upon protein aggregation in the cell
Rui Sun1,2, Wang Wan1, Wenhan Jin1
1CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, China. liuyu@dicp.ac.cn.
Abstract:
Protein aggregation in the cell is often manifested by the formation of subcellular punctate structures. Herein, we modulated the solvatochromism and solubility of Nile Red fluorophore derivatives to quantitatively study the polarity inside pathogenic protein aggregates, revealing structure- and protein-dependent polarity heterogeneity.

