Effect of sulfamethoxazole on nitrate removal by simultaneous heterotrophic aerobic denitrification

The increase in mariculture activities worldwide has not only led to a rise of nitrogen compounds in the ecosystem but has also intensified the accumulation of antibiotics in both terrestrial and marine environments. This study focused on the effect of typical antibiotics, specifically sulfamethoxazole (SMX) on nitrate removal from mariculture wastewater by aerobic denitrification process; an aerobic denitrification system feeding with 148.2 mg/L COD, 8.59 mg/L nitrate, 0.72 mg/L nitrite, and 4.75 mg/L ammonium was set up. The hydraulic retention time (HRT) was 8 h. As the aerobic bioreactor started up successfully without SMX dosage, an excellent removal of ammonium, nitrite, and nitrate was achieved at 91.35%, 93.33%, and 88.51%, respectively; the corresponding effluent concentrations were 0.41 mg/L, 0.048 mg/L, and 0.96 mg/L. At the influent SMX doses of 0, 1, 5, and 10 mg/L, the COD removal reached 96.91%, 96.27%, 88.69%, and 85.89%, resulting in effluent concentrations of 4.53, 5.45, 17.38, and 20.6 mg/L, respectively. Nitrification was not inhibited by SMX dosage. However, aerobic denitrification was inhibited by 10 mg/L SMX. Proteobacteria was the most abundant phylum, and surprisingly its abundance increased with the increase in SMX concentration. An excellent SMX degradation was noted at initial SMX dosages of 1, 5, and 10 mg/L; the removal rate was 100%,100%, and 99.8%, respectively. The SMX degrading genera Comamonas sp., Acinetobacter sp., and Thauera sp. are of great validity to wastewater engineers because they have demonstrated efficiency in simultaneous heterotrophic aerobic denitrification and antibiotic degradation as well as COD removal. PRACTITIONER POINTS: Nitrification was not inhibited by increase in SMX dosage. An increase in SMX dosage inhibited aerobic denitrification. COD removal was not affected by increased SMX dosage. Comamonas, Acinetobacter, and Thauera had high efficiency in COD removal and SMX degradation.