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Related Experiment Video

Updated: Sep 27, 2025

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

Published on: June 14, 2018

An optimized platform for efficient siRNA delivery into human NK cells.

Daniel Palacios^1,2, Pouria Momayyezi³, Oisín Huhn³

¹Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.

European Journal of Immunology

|April 13, 2022

Summary

Researchers developed a new method for delivering small interfering RNA (siRNA) into natural killer (NK) cells. This technique allows for efficient gene function studies in human NK cells without harming them.

Keywords:

Gene regulation Molecular biology NK cells

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Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

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Last Updated: Sep 27, 2025

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

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Published on: June 14, 2018

Engineering Chimeric Antigen Receptor-Natural Killer Cells Targeting Fungal Infections Using the Non-viral Sleeping Beauty Transposon System

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Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Published on: January 15, 2018

Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

The molecular mechanisms governing natural killer (NK) cell functions are not fully elucidated.
Understanding these networks is crucial for advancing immunotherapy and treating diseases.

Purpose of the Study:

To establish an efficient and viable method for delivering small interfering RNA (siRNA) into primary human NK cells.
To enable rapid functional genomics studies in human NK cells.

Main Methods:

Development of a novel workflow for siRNA delivery into human NK cells.
Assessment of cell viability post-transfection.
Application of the method for gene function interrogation.

Main Results:

The presented workflow achieves efficient siRNA delivery into human NK cells.
The method ensures high cell viability, preserving NK cell integrity.
This approach facilitates prompt investigation of gene functions in primary human NK cells.

Conclusions:

The developed siRNA delivery workflow is a valuable tool for NK cell research.
This methodology significantly advances the ability to study gene function in primary human NK cells.
This technique holds promise for accelerating discoveries in NK cell biology and related therapeutic applications.