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Related Concept Videos

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Related Experiment Video

Updated: Sep 27, 2025

Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
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A single tick screening for infectious pathogens using targeted mass spectrometry.

Holly R Smith1,2, Emily H Canessa1,3, Runia Roy1

  • 1Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, SUNY Binghamton University, Binghamton, NY, USA.

Analytical and Bioanalytical Chemistry
|April 13, 2022
PubMed
Summary

Black-legged ticks carry various pathogens, including Lyme disease bacteria. Proteomics identified Rickettsia and Borrelia species in ticks from New York, revealing high infection rates and aiding pathogen-tick interaction studies.

Keywords:
BorreliaLyme diseaseMass spectrometryProteomicsRickettsiaTicks

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Area of Science:

  • Entomology
  • Microbiology
  • Proteomics

Background:

  • Black-legged ticks (Ixodes scapularis) transmit Lyme disease (Borrelia burgdorferi) and other pathogens like Rickettsia and Anaplasma.
  • Assessing pathogen prevalence in ticks is crucial for understanding disease transmission and tick-pathogen interactions.

Purpose of the Study:

  • To screen black-legged ticks from Southern Tier, New York, for specific pathogen protein signatures using mass spectrometry-based proteomics.
  • To determine the prevalence of key bacterial pathogens within tick populations in the studied region.

Main Methods:

  • Bottom-up proteomic analysis of pooled tick samples to identify tick and pathogen proteins.
  • Parallel Reaction Monitoring (PRM) on individual tick samples to quantify specific pathogen proteins.
  • Data-dependent acquisition (DDA) for initial protein signature discovery.

Main Results:

  • Identification of 2,052 tick proteins and 41 pathogen proteins with high confidence.
  • High spectral match counts for Rickettsia and Borrelia species, with lower counts for Anaplasma phagocytophilum.
  • PRM analysis revealed 80% of individual ticks harbored Rickettsia, 50% harbored Borrelia, and 30% carried both.

Conclusions:

  • Mass spectrometry proteomics is a specific method for detecting pathogen proteins in ticks.
  • This approach provides insights into tick-pathogen interactions at the molecular level, particularly surface protein expression.
  • The study highlights significant Rickettsia and Borrelia co-infection rates in black-legged ticks from New York.