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Related Concept Videos

Heterochromatin02:38

Heterochromatin

14.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
14.8K
Euchromatin01:01

Euchromatin

7.7K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
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Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

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Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

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The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.6K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

6.1K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
6.1K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

23.8K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
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Updated: Sep 27, 2025

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
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Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

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Does chromatin function as a metabolite reservoir?

Vinícius D Nirello1, Dieggo Rodrigues de Paula1, Nathália V P Araújo1

  • 1International Laboratory for Microbiome Host Epigenetics, Department of Genetics, Evolution, Microbiology, and Immunology, Institute of Biology, University of Campinas, Campinas, SP, Brazil.

Trends in Biochemical Sciences
|April 14, 2022
PubMed
Summary
This summary is machine-generated.

Histone acylations link gene expression to cell metabolism. New insights suggest these histone post-translational modifications directly regulate metabolite pools, offering a new perspective on their roles.

Keywords:
acyl-CoAacylationschromatinhistone modificationsmetabolismquiescence

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The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
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Last Updated: Sep 27, 2025

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The ChroP Approach Combines ChIP and Mass Spectrometry to Dissect Locus-specific Proteomic Landscapes of Chromatin
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Epigenetics

Background:

  • Histone modifications, such as acylations, are known to regulate gene expression.
  • Cellular metabolic states influence the availability of acyl-coenzyme A (acyl-CoA) species.
  • Recent studies suggest a direct link between metabolite pools and histone post-translational modifications (PTMs).

Purpose of the Study:

  • To explore the integration of gene expression with cellular metabolic states through alternative histone acylations.
  • To investigate the potential for histone PTMs to directly regulate metabolite pools.
  • To provide a metabolite-centric view on the roles and evolution of histone PTMs.

Main Methods:

  • Analysis of cellular acyl-CoA pools.
  • Measurement of histone acylation levels.
  • Integration of gene expression data with metabolic profiles.

Main Results:

  • Alternative histone acylations were found to correlate with cellular metabolic states.
  • Evidence suggests histone PTMs directly impact metabolite pool composition.
  • A metabolite-centric perspective offers novel insights into histone PTM regulation.

Conclusions:

  • Histone acylations serve as a crucial interface between gene expression and cellular metabolism.
  • Histone PTMs are not only regulated by metabolites but also actively participate in their regulation.
  • Understanding this interplay provides a new framework for studying epigenetics and metabolic diseases.