Related Experiment Video
Updated: Sep 27, 2025
08:34
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
Published on: June 3, 2016
15.3K
CHD7 regulates bone-fat balance by suppressing PPAR-γ signaling
Caojie Liu1, Qiuchan Xiong1, Qiwen Li1
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, PR China.
Nature Communications
|April 14, 2022
Summary
Chromodomain helicase DNA-binding protein 7 (CHD7) deficiency in bone marrow stem cells causes low bone mass and high marrow fat. Loss of CHD7 disrupts epigenetic regulation of adipogenesis, impacting skeletal health.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- Chromodomain helicase DNA-binding protein 7 (CHD7) is crucial for organ development and its mutation causes CHARGE syndrome.
- The role of CHD7 in skeletal development and its underlying mechanisms are not fully understood.
Purpose of the Study:
- To investigate the function and epigenetic mechanisms of CHD7 in skeletal homeostasis.
- To elucidate the impact of CHD7 deficiency on bone marrow mesenchymal stem cells (MSCs) and their differentiation.
Main Methods:
- Conditional knockout of Chd7 in mouse bone marrow MSCs and preosteoblasts.
- Analysis of bone mass, marrow adiposity, and gene expression.
- Investigation of peroxisome proliferator-activated receptor (PPAR) signaling pathway and epigenetic modifications.
Main Results:
- Chd7 knockout in MSCs resulted in significantly reduced bone mass and increased marrow adiposity.
- Loss of CHD7 enhanced PPAR signaling, leading to increased PPAR-γ activity.
- PPAR-γ associated with H3K4me3, activating adipogenic genes and disrupting the osteogenic/adipogenic balance.
Conclusions:
- CHD7 plays a critical role in maintaining skeletal health by regulating MSC differentiation.
- Epigenetic dysregulation of PPAR signaling due to CHD7 loss contributes to skeletal pathologies.
- Findings reveal a novel epigenetic mechanism linking CHD7 to skeletal diseases.
More Related Videos
Related Concept Videos
01:29Cholesterol: Significance and Regulation
720
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
Considering cholesterol and...
720
01:17Hormones and Bone Tissue
3.0K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.0K
01:58Cell Specific Gene Expression
14.1K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.1K
01:40Bone Remodeling
38.6K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.6K
02:33Hedgehog Signaling Pathway
7.6K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.6K
01:09GPCRs Regulate Adenylyl Cylase Activity
6.0K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.0K