Biological reference interval of nitric oxide in health and disease

There is a need to develop reference interval of NO in health and disease.

Subjects aged between 25 and 55 years were drawn from a random sample of the north Indian population, based on defined inclusion and exclusion criteria. Measurement of NO was done based on principle of greiss reaction.

Reference interval of NO in healthy individual of age group 25-55 years was 21 ± 13.3 μM/L (n=350), Premenopausal women of age group 25-35 years was 12.7 ± 4.9 μM/L (n=180), Postmenopausal women of age group 40-55 years was 10.3 ± 3.84 μM/L (n=100) and healthy pregnant females of age group 25-35 years was 70.9 ± 15.95 μM/L (n=330). In pathological state group, reference interval of NO in metabolic syndrome cases of age group 25-55 years was 19.4 ± 15.3 μM/L (n=100), coronary artery disease patient of age group more than 35 years was 17.6 ± 10.8 μM/L (n=160), pregnancy induced hypertension (PIH) of age group 25-30 years was 45.7 ± 7.2 μM/L (n=330), pre-eclampsia patient of age group 25-35 years was 39.8 ± 14.7 μM/L (n=200) and diabetic individuals of age group of more than 30 years was 15.5 ± 1.4 μM/L (n=50).

The reference intervals presented may be used for various research purposes. Based upon our study, reference interval for NO levels of various disease states like MetS, CAD, diabetes, PIH showed lower levels of NO compared to their respective healthy group due to shared etiopathologies with decreased NO levels.