Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

8.1K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
8.1K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

3.2K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.2K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

3.8K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Data-Independent Acquisition for Improved Compound Annotation in MALDI MS Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same author

ADAR1 loss-of-function variants altering RNA editing define a new interferon-dependent psoriasis subtype.

The Journal of experimental medicine·2026
Same author

Beyond the Fixed Charge: A New On-Tissue Derivatisation Strategy Employing Photoionisable Chromophores for Mass Spectrometry Imaging of Amino-Containing Metabolites.

Angewandte Chemie (International ed. in English)·2026
Same author

GPCS Stratification of Exercise-Induced Gut Microbiota and Metabolome Remodeling in IBS: An Exploratory Multi-Omics Study.

Nutrients·2026
Same author

Caged Monomethyl Auristatin F (MMAF) for Cell-Specific Activation Using a 488-nm-Optimized Photolabile Group.

JACS Au·2026
Same author

Dynamic neutrophil lipidome remodeling during induction of NETosis.

Science advances·2026

Related Experiment Video

Updated: Sep 27, 2025

Murine Dermal Fibroblast Isolation by FACS
06:04

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

21.5K

Sphingolipids control dermal fibroblast heterogeneity.

Laura Capolupo1, Irina Khven2, Alex R Lederer2

  • 1Interfaculty Institute of Bioengineering and Global Health Institute, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

Science (New York, N.Y.)
|April 14, 2022
PubMed
Summary

Cellular lipid differences are crucial for skin structure. Sphingolipid variations define fibroblast states, influencing cell behavior and multicellular organization.

More Related Videos

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.1K
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

9.8K

Related Experiment Videos

Last Updated: Sep 27, 2025

Murine Dermal Fibroblast Isolation by FACS
06:04

Murine Dermal Fibroblast Isolation by FACS

Published on: January 7, 2016

21.5K
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.1K
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
12:04

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

9.8K

Area of Science:

  • Cellular Biology
  • Lipidomics
  • Systems Biology

Background:

  • Human cells generate thousands of lipids, with composition varying during differentiation and between individual cells.
  • The functional significance of cell-to-cell lipid heterogeneity remains largely uncharacterized.

Purpose of the Study:

  • To investigate the functional role of lipid heterogeneity in human dermal fibroblasts.
  • To link individual cell lipid profiles with their transcriptional states and contribution to tissue organization.

Main Methods:

  • Coupling of high-resolution mass spectrometry imaging with single-cell transcriptomics.
  • Analysis of lipidomes and transcriptomes from individual human dermal fibroblasts.

Main Results:

  • Cell-to-cell variations in lipid metabolic pathways were found to influence cell states related to skin architecture.
  • Sphingolipid composition was identified as a key determinant of fibroblast subpopulations.
  • Sphingolipid metabolic rewiring was observed to drive transitions between cell states.

Conclusions:

  • Cell-to-cell lipid heterogeneity plays a significant role in determining cell states.
  • Lipid heterogeneity adds a novel regulatory layer to the self-organization of multicellular systems.