Nitrogen removal by a novel heterotrophic nitrification and aerobic denitrification bacterium Acinetobacter
Linhui Wu1, Xiaoyu Ding1, Yan Lin1
1School of Ecology and Environment, Inner Mongolia University, Hohhot 010021, China.
Abstract:
A new bacterial strain, Acinetobacter calcoaceticus TY1, was identified in activated sludge. This strain efficiently metabolized nitrogen from ammonium at low temperatures, utilizing NH4+-N, NO3--N, and NO2--N as nitrogen sources. Of these, NH4+-N was superior in terms of both assimilation and heterotrophic nitrification at 8 °C. The nitrogen metabolism-associated genes amoA, nirK, and nosZ were identified in TY1. Optimal requirements for growth and nitrogen removal were pH 7, shaking speed of 90 rpm, a C/N ratio of 10, and sodium citrate for the carbon supply. The ability to denitrify at low temperature suggests TY1's potential for wastewater management.
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