On-line separation/analysis of Rhodamine B dye based on a solid-phase extraction high performance liquid...

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Rhodamine B (RhB) is a widely used dye with potential environmental and health concerns.
• Accurate and sensitive detection methods for RhB are crucial for environmental monitoring and quality control.

Purpose of the Study:

• To develop a novel on-line solid-phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC) method for Rhodamine B (RhB) dye analysis.
• To utilize a self-designed device based on poly-1-vinyl-3-pentylimidazole hexafluorophosphate (PILs-C5) for RhB separation and detection.

Main Methods:

• Optimization of experimental parameters including pH, PILs-C5 amount, sample volume, and eluent conditions using single factor and orthogonal designs.
• On-line solid-phase extraction (SPE) using PILs-C5 material.
• High-performance liquid chromatography (HPLC) for separation and detection of RhB.

Main Results:

• Achieved a linear range of 0.02-2.4 μg mL⁻¹ with a high correlation coefficient (R² = 0.997).
• Obtained a low limit of detection (LOD) of 0.004 μg mL⁻¹ and limit of quantification (LOQ) of 0.02 μg mL⁻¹.
• Demonstrated a high extraction capacity (6.22 mg g⁻¹) and enrichment ratio (15) for RhB.

Conclusions:

• The developed PILs-C5 based on-line SPE-HPLC method is highly effective for sensitive and accurate Rhodamine B dye determination.
• The method shows excellent performance and applicability for analyzing RhB in diverse real-world samples.