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Controlled "off-on" fluorescent probe for the specific detection of hyperhomocysteinemia
Jinrong Zheng1, Jianlong Li2, Hongli Luo3
1Xiamen Cardiovascular Hospital, Xiamen University Xiamen 361006 China.
Insights
A new fluorescent probe specifically detects homocysteine (Hcy), a risk factor for cardiovascular disease. This tool offers efficient in vivo and in vitro detection of hyperhomocysteinemia for early disease warning.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Cardiovascular Research
Background:
- Hyperhomocysteinemia is a significant risk factor for atherosclerosis and vascular diseases.
- Early detection of hyperhomocysteinemia is crucial for preventing cardiovascular diseases.
- Developing specific probes for homocysteine (Hcy) presents a considerable challenge.
Purpose of the Study:
- To design and develop a highly selective and sensitive fluorescent probe for homocysteine.
- To evaluate the probe's anti-interference capabilities against other biological molecules.
- To assess the probe's efficacy in detecting hyperhomocysteinemia in vitro and in vivo.
Main Methods:
- Design and synthesis of a novel homocysteine-specific fluorescent probe (HSFP).
- Spectroscopic analysis to determine probe selectivity and sensitivity towards Hcy.
- Testing the probe's performance in aqueous solutions with potential interfering substances.
- Evaluation of HSFP's ability to detect Hcy changes within cells.
- Application of HSFP for in vivo detection of hyperhomocysteinemia.
Main Results:
- The developed HSFP exhibits excellent selectivity and anti-interference capabilities.
- HSFP shows a significant fluorescence attenuation (13.8-fold) in the presence of Hcy in aqueous solutions.
- The probe effectively detects changes in Hcy content within cells.
- HSFP demonstrates high efficiency in detecting hyperhomocysteinemia in vivo.
Conclusions:
- A novel Hcy-specific fluorescent probe (HSFP) has been successfully developed.
- HSFP offers efficient and selective detection of Hcy in vitro and in vivo.
- This probe holds potential for early warning and research in cardiovascular diseases related to hyperhomocysteinemia.
Abstract:
Hyperhomocysteinemia is an established risk factor for atherosclerosis and vascular disease. Therefore, designing a hyperhomocysteinemia specific probe is of great significance for the early warning of cardiovascular diseases. However, developing probes that can efficiently and specifically recognize homocysteine (Hcy) remains a tremendous challenge. Therefore, we designed an Hcy-specific fluorescent probe (HSFP) with excellent selectivity and anti-interference capability. Interestingly, this probe can automatically "off-on" in water solution, but the fluorescence of HSFP remains "off" when Hcy is present in the solution. The spectroscopic data demonstrated that the fluorescence of HSFP attenuated 13.8 folds toward Hcy in water without interference from other biothiols and amino acids. Furthermore, HSFP can sensitively reflect the change of Hcy content in cells. Therefore, HSFP was further applied to detect hyperhomocysteinemia in vivo with high efficiency. In summary, we have developed an Hcy-specific fluorescent probe to efficiently detect Hcy in vivo and in vitro, which may contribute to basic or clinical research.
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