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Updated: Sep 27, 2025
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
A dual responsive fluorescent probe for selective detection of cysteine and bisulfite and its application in
Xiaofeng Wang1, Mingshun Li2, Tingting Duan1
1Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Hainan Medical University Haikou 570102 China xuejunzhouent@hainmc.edu.cn denieceduan@163.com zouyuxiazzz@163.com.
Abstract:
A coumarin-based dual responsive fluorescent probe with a simple structure was developed for the detection of Cys and HSO3 -. Under simulated physiological conditions, Cou-F displayed an on-off fluorescence response to Cys at 521 nm and an off-on fluorescence response to HSO3 - at 500 nm. Furthermore, Cou-F had the advantages of high sensitivity, strong specificity and rapid response. The detection limits of Cou-F toward Cys and HSO3 - were 0.54 μM and 0.65 μM, respectively. Cou-F enabled high selective responses to Cys and HSO3 - over other biologically related species. The response times of Cou-F toward Cys and HSO3 - were 80 s and 100 s. The fluorescence imaging of Cys and HSO3 - was achieved in living RAW246.7 cells.
