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Updated: Sep 27, 2025
3D Magnetic Stem Cell Aggregation and Bioreactor Maturation for Cartilage Regeneration
Published on: April 27, 2017
[Research progress of different cell seeding densities and cell ratios in cartilage tissue engineering]
Huifeng Xie1,2, Wei Zhou1,2, Bo Bai1,2
1Department of Orthopedics, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Guangdong, 510120, P. R. China.
Objective:
To review the research progress of different cell seeding densities and cell ratios in cartilage tissue engineering.
Methods:
The literature about tissue engineered cartilage constructed with three-dimensional scaffold was extensively reviewed, and the seeding densities and ratios of most commonly used seed cells were summarized.
Results:
Articular chondrocytes (ACHs) and bone marrow mesenchymal stem cells (BMSCs) are the most commonly used seed cells, and they can induce hyaline cartilage formation in vitro and in vivo. Cell seeding density and cell ratio both play important roles in cartilage formation. Tissue engineered cartilage with good quality can be produced when the cell seeding density of ACHs or BMSCs reaches or exceeds that in normal articular cartilage. Under the same culture conditions, the ability of pure BMSCs to build hyaline cartilage is weeker than that of pure ACHs or co-culture of both.
Conclusion:
Due to the effect of scaffold materials, growth factors, and cell passages, optimal cell seeding density and cell ratio need further study.
