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Related Experiment Video

Updated: Sep 27, 2025

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy
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Theory of defect-mediated morphogenesis.

Ludwig A Hoffmann1, Livio Nicola Carenza1, Julia Eckert2

  • 1Instituut-Lorentz, Universiteit Leiden, P.O. Box 9506, 2300 RA, Leiden, Netherlands.

Science Advances
|April 15, 2022
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Summary
This summary is machine-generated.

Topological defects in active liquid crystals drive tissue morphogenesis by causing instability and protrusions. This research explains how these defects organize cellular layers, leading to complex shape changes and active turbulence.

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Area of Science:

  • Physics
  • Biophysics
  • Materials Science

Background:

  • Topological defects are increasingly recognized for their role in organizing biological tissues during development.
  • Understanding the physical mechanisms underlying tissue morphogenesis is crucial for developmental biology and regenerative medicine.

Purpose of the Study:

  • To provide a quantitative explanation for how topological defects act as organizing centers in tissue morphogenesis.
  • To investigate the role of active polar liquid crystals and buckling theory in this process.

Main Methods:

  • Linear stability analysis
  • Computational fluid dynamics simulations
  • Modeling of confined cell monolayers as active layers

Main Results:

  • Active layers with topological defects (disclinations) are unstable to protrusion formation.
  • Instability arises from the interplay of elastic forces focused by defects and active flow-induced surface tension renormalization.
  • The post-transitional regime exhibits complex morphodynamical behaviors including oscillations, droplet nucleation, and active turbulence.

Conclusions:

  • Topological defects quantitatively explain organizing centers in tissue morphogenesis.
  • The findings elucidate the dynamics of active surfaces and provide a framework for understanding experimental observations in tissue development.