Investigating role of abiotic side and finding optimum abiotic condition for improving gas biodesulfurization using Thioalkalivibrio versutus

Hydrogen sulfide (H₂S) is a super toxic substance that produces SO_x gases when combusted. Therefore, it should be removed from gas streams. Biodesulfurization is one of the developing methods for removing sulfide. Gas biodesulfurization must be accelerated to be competitive with chemical processes. This process has two sides: biotic and abiotic sides. To increase the rate of sulfide removal, this substance should be given to the bacteria in the maximum amount (Max. - R_{HS B}). Therefore, it is necessary to minimize the rate of adverse abiotic reactions of sulfide (Min. - R_{HS A}). Minimizing the sulfide reaction with biosulfur and oxygen and thiosulfate generation (Min. - R_{HS thio2}) was assessed in de-microbized medium. It was concluded that the pH should be kept as low as possible. The kinetics of thiosulfate formation from sulfide oxidation (- R_{HS thio1}) are strongly dependent on the sulfide concentration, and to minimize this reaction rate, sulfide should be gently injected into the culture. To minimize sulfide reduction to hydrogen sulfide (Min. - R_{HS rev}), the pH should be kept as high as possible. Using the Design Expert v.13, a model was driven for the abiotic side to obtain optimum condition. The pH value was found to be 8.2 and the sulfide concentration to 2.5E-05 M. Thioalkalivibrio versutus cultivation under identified abiotic conditions resulted in biological removal of sulfide up to 1.5 g/h. The culture was not able to remove 2 g/h input sulfide, and to increase this, the biotic side should be studied.