A novel hydrogen peroxide fluorescent probe for bioimaging detection and enables multiple redox cycles

In this subject, a novel hydrogen peroxide (H₂O₂) fluorescent probe (MNG) was designed and developed using naphthalimide derivatives and selenomorpholine. In PBS buffer (10 mM, pH = 7.4, 1 %DMSO), the selenomorpholine on the probe is capable of qualitatively and quantitatively detecting (H₂O₂) at a small amount under a detection limit of 61 nM. The probe follows a mechanism that Se (Ⅱ) in selenomorpholine is transformed to Se (Ⅳ), thus changing the spectra of the probe MNG. It is noteworthy that MNG can continuously make a cyclic response to H₂O₂ and glutathione (GSH), so it can potentially achieve redox process imaging in vivo. Moreover, this subject verified the redox process of the probe's continuous redox response in the Gaussian 09 programme through simulation calculation and mass spectrometry. The probe exhibits high biocompatibility. Moreover, it can detect H₂O₂ in MCF-7 cells and Argentine Bloodfin.