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CTL adhesion and antigen recognition are discrete steps in the human CTL-target cell interaction
Journal of Immunology (Baltimore, Md. : 1950)
|March 1, 1987
Summary
Cytolytic T lymphocyte (CTL) adhesion to target cells occurs independently of antigen recognition. Specific HLA molecules and cell adhesion are both required for efficient CTL activation and target cell lysis.
Area of Science:
- Immunology
- Cell Biology
Background:
- Cytolytic T lymphocyte (CTL)-mediated killing is crucial for immune response.
- Initial CTL-target cell interaction involves adhesion and antigen recognition.
Purpose of the Study:
- To investigate the initial events in CTL-target cell interaction.
- To determine the role of antigen-specific receptors and cell adhesion in CTL activation.
Main Methods:
- Used HLA-A2- and HLA-B7-specific human CTL clones and HLA-typed B lymphoblastoid target cells.
- Employed adhesion assays and flow cytometry with calcium-sensitive dye Indo-1.
- Conducted MAb inhibition studies.
Main Results:
- Demonstrated antigen-nonspecific CTL-target cell adhesion, independent of T cell receptor recognition.
- Showed that CTL-target cell adhesion alone does not activate CTL clones.
- Found that both adhesion and the presence of appropriate HLA target molecules are necessary for efficient CTL activation.
Conclusions:
- Antigen-nonspecific adhesion molecules (LFA-1, CD2, LFA-3) regulate CTL-target cell interaction.
- These molecules facilitate antigen recognition and subsequent target cell lysis by CTLs.