Quantitation of cell-surface beta 2-microglobulin (beta 2 m) using a comparative antibody inhibition assay

An improved immunoenzyme assay for measuring cell-surface beta 2-microglobulin (beta 2 m) is presented in which quantitation is achieved by reference to soluble beta 2 m-induced inhibition of horseradish peroxidase-labelled rabbit antibody. Some consideration is given to kinetics of binding and dissociation, and their implications for interpretation are discussed.