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Updated: Sep 26, 2025
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
All on size-coded single bead set: a modular enrich-amplify-amplify strategy for attomolar level multi-immunoassay
Desheng Chen1,2, Xiaobo Zhang1, Liping Zhu1
1Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University Xi'an 710119 Shaanxi Province P. R. China liuch@snnu.edu.cn.
We developed an ultrasensitive immunoassay using Single Bead Enrich-Amplify-Amplify (SBEAA) to detect low-level protein biomarkers. This method enhances signals on a single bead, enabling accurate quantification for early diagnosis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Ultrasensitive protein analysis is crucial for early disease diagnosis and biological research.
- Quantifying low-abundance protein biomarkers (aM to fM) is challenging due to weak signals masked by background noise.
Purpose of the Study:
- To develop a novel ultrasensitive immunoassay for accurate quantification of low-concentration protein biomarkers.
- To overcome the limitations of existing methods in detecting trace protein levels.
Main Methods:
- A modular Single Bead Enrich-Amplify-Amplify (SBEAA) strategy was employed.
- In situ signal amplification was achieved using a cascade tyramide signal amplification system.
- A size-coded SBEAA set (SC-SBEAA) was designed for multiplexed assays.
Main Results:
- The SBEAA strategy achieved highly efficient target enrichment on a single bead, confining signal output.
- Cascade tyramide signal amplification significantly enhanced the in situ signal, exceeding background noise.
- The SBEAA system demonstrated a wide dynamic range (aM to nM) and modularity for varied clinical needs.
Conclusions:
- The proposed SBEAA immunoassay offers ultrasensitive and accurate protein quantification, overcoming background interference.
- The modularity and multiplexing capabilities of SBEAA, including SC-SBEAA, provide versatile solutions for clinical diagnostics and rare sample analysis.
