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Updated: Sep 26, 2025

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

An optimized protocol for phenotyping human granulocytes by mass cytometry.

Nora Vivanco Gonzalez¹, John-Paul Oliveria^1,2, Dmitry Tebaykin¹

¹Department of Pathology, School of Medicine, Stanford University, Stanford, CA, United States.

|April 18, 2022

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This study presents a mass cytometry (CyTOF) protocol to analyze human granulocytes, including basophils, eosinophils, and neutrophils. The method allows detailed characterization of these immune cells under various conditions.

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Granulocytes play critical roles in pathogen defense and allergic inflammation.
Distinct granulocyte phenotypes are associated with their diverse immune functions.
Understanding granulocyte heterogeneity is crucial for immune response research.

Purpose of the Study:

To develop and optimize a mass cytometry (CyTOF) protocol for comprehensive granulocyte analysis.
To enable simultaneous evaluation of human basophils, eosinophils, and neutrophils.
To characterize granulocyte phenotypes under homeostatic and immune-activated conditions.

Main Methods:

Utilized mass cytometry (CyTOF) for high-dimensional single-cell analysis.
Optimized protocols for granulocyte integrity and protein marker detection.

Keywords:

Antibody Cell isolation Flow Cytometry/Mass Cytometry Health Sciences Immunology Single Cell

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

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Last Updated: Sep 26, 2025

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

Published on: June 19, 2019

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Sample Preparation for Mass Cytometry Analysis

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Applied the method to human granulocytes stimulated with anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3).

Main Results:

Successfully captured distinct cellular phenotypes of human granulocytes.
Enabled deep characterization of both common and rare granulocyte populations.
Demonstrated the protocol's utility in analyzing immune cell responses.

Conclusions:

The developed CyTOF protocol provides a robust method for detailed granulocyte profiling.
This technique facilitates the study of granulocyte heterogeneity and function in health and disease.
The protocol supports research into allergic inflammation and immune activation.