Hydrogenophaga crocea sp. nov. associated with cyanobacterial mat isolated from farmland mud

A catalase and oxidase-positive strain BA0156^T was isolated from a cyanobacterial mat collected from the farmland mud cultivated with sugarcane from Ahmednagar, India. The 16S rRNA gene of strain BA0156^T showed the highest percent sequence similarity with Hydrogenophaga borbori LMG 30805^T (98.5%), followed by H. flava DSM 619^T (98.3%) and H. intermedia DSM 5680^T (98.2%). The strain BA0156^T contained the major fatty acids, C_16:0 (25.1%) and C_17:0 cyclo (3.9%), whereas phosphatidylethanolamine and diphosphatidylglycerol were the major polar lipids. The OrthoANI and dDDH values between strain BA0156^T and its closest relative H. borbori LMG 30805^T were 84.6% and 28.3%, respectively. The DNA G+C content of strain BA0156^T was 69.4 mol %. Furthermore, the biochemical and physiological features of strain BA0156^T showed a distinct pattern from their closest phylogenetic neighbours. The phenotypic, genotypic and chemotaxonomic characteristics indicated that the strain BA0156^T represents a new species for which the name Hydrogenophaga crocea (type strain BA0156^T = MCC 3062^T = KCTC 72452^T = JCM 34507^T) is proposed.