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Updated: Sep 26, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Draft Genome Sequence of a Novel Iflavirus from Leafhoppers (Exitianus capicola) in Iraq
Zhuang-Xin Ye1, Adnan A Lahuf2, Mohammed D Salman2
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Abstract:
The draft genome sequence of a novel virus was determined from a leafhopper insect (Exitianus capicola) via transcriptomic sequencing and rapid amplification of cDNA ends. Results of the sequence alignment suggested that the new virus is a member of the genus Iflavirus, family Iflaviridae. Thus, it was proposed to be named Exitianus capicola iflavirus 2 (ECIV2). ECIV2 has a single positive-strand RNA genome of 7,821 nucleotides [excluding the poly(A) tail] containing a single open reading frame (ORF) with typical iflavirus conserved domains, including a picornavirus capsid protein-like domain, a cricket paralysis virus capsid-like domain, a helicase, a peptidase, and an RNA-dependent RNA polymerase domain.

