Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Delivery: Overview01:16

Drug Delivery: Overview

444
The selection of a drug's delivery route depends upon its physicochemical properties, including lipid or water solubility and ionization, as well as the therapeutic requirement, such as immediate or sustained effect. These routes can be divided into three primary categories: enteral, parenteral, and topical.
Enteral delivery involves administering drugs directly through swallowing, sublingual placement, or buccal application. Orally administered drugs predominantly navigate the...
444
Overview of Exosomes01:36

Overview of Exosomes

2.9K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exosome Nanotechnology in Molecular Medicine Advances, Applications and Challenges in Gene Therapy.

Expert reviews in molecular medicine·2026
Same author

Ear Cartilage Reconstruction: From Regenerative Medicine to Reconstructive Surgery.

Current stem cell research & therapy·2026
Same author

Evaluating immunoreactivity of hybridoma-derived antibody mixtures generated against AU-565 cell line for diagnosis and immunotherapy of breast cancer.

BMC cancer·2026
Same author

Adjuvant choice shapes macrophage activation and cytokine responses to freeze-thaw breast cancer antigens.

BMC research notes·2026
Same author

Complete Healing After Umbilical Mesenchymal Stem Cell-Derived Exosome Therapy For a Refractory Complex Anal Fistula.

Cureus·2026
Same author

Poly(ε-caprolactone) Nanoparticle Tumor-Lysate Vaccination in Mice Generates Hybridoma-Derived Antibodies Enabling Breast Cancer Diagnosis and Chemotherapy Synergy.

Biomedicines·2026

Related Experiment Video

Updated: Sep 26, 2025

Formulating and Characterizing an Exosome-based Dopamine Carrier System
06:08

Formulating and Characterizing an Exosome-based Dopamine Carrier System

Published on: April 4, 2022

3.3K

Formulating and Characterizing an Exosome-based Dopamine Carrier System.

Burçak Yavuz1, Hakan Darici2, Aslı Pınar Zorba Yildiz3

  • 1Vocational School of Health Services/İstanbul, Altinbas University; Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University; burcakyavuzz@gmail.com.

Journal of Visualized Experiments : Jove
|April 18, 2022
PubMed
Summary

Researchers developed a novel nanocarrier by loading dopamine into exosomes derived from stem cells. These dopamine-loaded exosomes show potential for treating central nervous system diseases by crossing the blood-brain barrier.

More Related Videos

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
07:30

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Published on: November 21, 2012

14.0K
Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
11:26

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

Published on: September 21, 2017

12.6K

Related Experiment Videos

Last Updated: Sep 26, 2025

Formulating and Characterizing an Exosome-based Dopamine Carrier System
06:08

Formulating and Characterizing an Exosome-based Dopamine Carrier System

Published on: April 4, 2022

3.3K
Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
07:30

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Published on: November 21, 2012

14.0K
Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
11:26

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

Published on: September 21, 2017

12.6K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cell Biology

Background:

  • Exosomes are small extracellular vesicles involved in intercellular communication.
  • Their ability to cross the blood-brain barrier suggests potential therapeutic applications in neurological diseases.
  • Mesenchymal stem cells offer a source for exosome isolation.

Purpose of the Study:

  • To develop an exosome-based nanocarrier system for drug delivery.
  • To encapsulate dopamine into exosomes derived from Wharton's jelly mesenchymal stem cells (WJ-MSCs).
  • To evaluate the feasibility and safety of dopamine-loaded exosomes.

Main Methods:

  • Isolation and characterization of exosomes from WJ-MSCs.
  • Incubation of exosomes with dopamine for encapsulation.
  • Recharacterization of dopamine-loaded exosomes.
  • Drug release and cytotoxicity assays were performed.

Main Results:

  • Successful encapsulation of dopamine within WJ-MSC-derived exosomes was confirmed.
  • Dopamine-loaded exosomes demonstrated controlled drug release properties.
  • The developed nanocarrier system exhibited no adverse effects on fibroblast viability.

Conclusions:

  • Exosomes can be effectively utilized as nanocarriers for drug delivery, specifically for dopamine.
  • Dopamine-loaded exosomes show promise as a safe and viable therapeutic strategy for central nervous system disorders.
  • This exosome-based system represents a significant advancement in nanomedicine for neurological conditions.