[HDL2 and HDL3 cholesterol in men and women in different forms of hyperlipidemia without arterial involvement]
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This study found that the cholesterol ratio decreased significantly in hyperlipidemic individuals. However, HDL2 cholesterol was higher in women, suggesting a protective role against atherosclerosis.
Area of Science:
- Cardiovascular Health
- Lipid Metabolism
- Biochemistry
Background:
- Hyperlipidemia is a significant risk factor for atherosclerosis.
- Understanding lipid profiles, particularly high-density lipoprotein (HDL) subclasses, is crucial for cardiovascular risk assessment.
- Gender-based differences in lipid metabolism may influence atherosclerosis development.
Purpose of the Study:
- To investigate lipid profiles in hyperlipidemic men and women compared to normolipidemic controls.
- To analyze the levels of HDL2 and HDL3 cholesterol and their ratios in different hyperlipidemia groups.
- To explore potential gender-specific protective mechanisms against atherosclerosis related to HDL cholesterol.
Main Methods:
- Lipoprotein separation using sequential ultracentrifugation.
- Isolation of HDL2 and HDL3 cholesterol at specific solvent densities.
- Enzymatic measurement of cholesterol levels.
- Comparison of lipid parameters between hyperlipidemic (WHO groups IIa, IIb, IV) and normolipidemic subjects, stratified by gender.
Main Results:
- A significant decrease in the (Formula: see text) ratio was observed in all hyperlipidemic subjects compared to controls.
- No significant variations in HDL2 and HDL3 cholesterol levels were found across hyperlipidemia groups versus controls.
- HDL2 cholesterol levels were significantly higher in hyperlipidemic women than in men, particularly in WHO groups IIa and IIb.
Conclusions:
- The (Formula: see text) ratio remains a key indicator for hyperlipidemia.
- Higher HDL2 cholesterol in women may indicate a protective effect against atherosclerosis.
- The (Formula: see text) ratio warrants further investigation for its potential role in hyperlipidemia assessment.
Abstract:
The investigation was carried out in one hand on 54 hyperlipidemic men without arterial injury compared to 54 normolipidemic men, on the other hand on 50 hyperlipidemic women compared to 50 normolipidemic women. The hyperlipidemic subjects were separated in IIa, IIb and IV groups, according to WHO classification. Lipoprotein's separation was carried out by sequently ultracentrifugation and HDL2-HDL3 were isolated at a solvant density of 1.125 and 1.21; cholesterol was measured by enzymatic method. We observed a significant decrease of: (Formula: see text) ratio in all hyperlipidemic subjects compared to controls; but no significant variation of HDL2 and HDL3 cholesterol appeared in the three groups of hyperlipidemic subjects compared to controls; on the other hand we noted a HDL2 cholesterol greater in women than in men and this fact seems to prove a favorable action of this fraction in preventing atherosclerosis. The (Formula: see text) ratio remains the most discriminant factor of hyperlipidemia, but the: (formula: see text) ratio seems interesting and HDL2 cholesterol is always higher in women during hyperlipidemia with high risk, IIa and IIb.