Related Experiment Video

Updated: Sep 26, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

14.0K

Correction: Best Practices in qPCR and dPCR Validation in Regulated Bioanalytical Laboratories

Amanda Hays1, Rafiq Islam2, Katie Matys3

  • 1BioAgilytix Labs, 2300 Englert Drive, Durham, NC, 27713, USA. Amanda.hays@bioagilytix.com.

The AAPS Journal
|April 19, 2022
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
07:43

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

Published on: July 14, 2023

2.5K
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

14.8K

Related Experiment Videos

Last Updated: Sep 26, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
08:37

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

14.0K
Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
07:43

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

Published on: July 14, 2023

2.5K
Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
08:54

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

Published on: November 5, 2020

14.8K

Related Concept Videos

Data Validation01:15

Data Validation

278
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
278

Articles linked to this work by shared authors, journal, and citation graph.

18th GCC Closed Forum: Compliance with FDA Biomarkers Guidance, Novel ELN/LIMS Platforms, Sample Reconciliation, Emerging Technologies, Improvements in ELISpot, Post-extraction Sample Stability, LBA Singlicate, Regulatory Inspections, Chicken anti-Human IgG, Cross-validation Harmonization.

Bioanalysis·2026

Progress and promise of pharmacodynamic biomarkers: novel strategies and assay considerations in drug development.

Bioanalysis·2026

2025 White Paper on Recent Issues in Bioanalysis: Biomarkers Calibrators & Stability; Evaluation of NULISA; Neurofilament & Autoantibody Biomarker Assays; Removing IgM Interference; ELISpot & FluoroSpot Best Practices; Modular HD Cytometry; Single-cell Analysis Imaging Cytometry (PART 2A - Recommendations on Biomarkers Discovery, Development, Validation & Regulatory Approval, Ligand-Binding Assays (LBA) and Cell-Based Assays (CBA) PART 2B - Regulatory Agencies' Input on Biomarkers, IVD/CDx and Biomarker Assay Validation (BAV)).

Bioanalysis·2026

Technical Considerations of Pharmacokinetic Assays for LNP-mRNA Drug Products by RT-qPCR.

The AAPS journal·2025

17th GCC Closed Forum: Integrative Bioanalysis, Patient-centric Sampling, Emerging Technologies, Data Integrity, Sample Reconciliation, Discrepancies of ELISpot Data, Cross-validation Harmonization, Ultrasensitive Platforms for ADA Assays, Remote Regulatory Assessments, Shedding Assays by PCR, and Biomarker Tissue Assays.

Bioanalysis·2025

Recommendations on biomarker assay validation (BAV) in tissues by GCC.

Bioanalysis·2025

Industry Perspective on Translational and Clinical Pharmacology Aspects of Viral-based Gene Therapies and Vaccines-Key Considerations and Learnings From Approved Products.

The AAPS journal·2026

A Machine Learning Platform for Imputing Missing Critical Quality Attributes in Pharmaceutical Development.

The AAPS journal·2026

Design, Optimization and Evaluation of Taste-Masked Acemetacin Formulations Using Purolite A430MR.

The AAPS journal·2026

An In Vitro Quantitative Systems Pharmacology Platform for Characterizing CD3-Bispecific Antibody-Mediated T-Cell Activation and Tumor Cell Cytotoxicity.

The AAPS journal·2026

Assessment of Sorbitol as a Pharmaceutical Excipient in Oral Liquid Pediatric Formulations Submitted to the U.S. FDA.

The AAPS journal·2026

Calculating Receptor Occupancy using TMDD Models - What has to be Considered?

The AAPS journal·2026

Analytical performance comparison of Siemens Atellica CI1900 and Roche Cobas Pure integrated analyzers across spectrophotometric, ion-selective electrode and immunoassay parameters.

Analytical biochemistry·2026

Operationalizing accreditation standards for laboratory-developed next-generation sequencing validation of CFTR amplicon-based assays.

Laboratory medicine·2026

What do laboratories know about the pneumatic tube delivery systems they depend on?

Biochemia medica·2026

Classification performance of the 2023 ACR/EULAR antiphospholipid syndrome criteria: a systematic review and meta-analysis.

Rheumatology (Oxford, England)·2026

Same sample, different result: Variation in clozapine assay measurement in four UK laboratories.

Therapeutic advances in psychopharmacology·2026

Mass Spectrometry-Based Anti-Doping Analysis: A Critical Review of Emerging Analytical and Sample Pretreatment Strategies.

Critical reviews in analytical chemistry·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us