A xanthene-based fluorescent probe for detection of peroxynitrite in living cells and zebrafish

Peroxynitrite (ONOO^-) is one of quite critical reactive oxygen species that acts critical roles in a number of diverse biological functions and pathological events. Notably, excessive ONOO^- will lead to sorts of diseases. Thus, monitoring of endogenous ONOO^- levels will be conducive to exploring the physiological activities and functions of ONOO^-. Here, a simple turn-on fluorescent probe named DMX is reported using CN bond as the ONOO^- recognition site and xanthene as the fluorophore. DMX possessed a good linear dependence with ONOO^- concentration (0-9 μM), highly sensitive detection (DL = 37 nM), and excellent selectivity towards ONOO^-. What is more, the biological experiments reveal that DMX is able to be utilized to track exogenous/endogenous ONOO^- employing confocal laser scanning microscopy. Visualization of ONOO^- in zebrafish was also successfully conducted, suggesting that DMX might be used to study ONOO^- roles in vivo. We believe that DMX will have potential for exploring the pivotal role of ONOO^- during all sorts of physiological and pathological activities.