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Published on: January 26, 2016
The CSY-protecting group in the microwave-assisted synthesis of aggregation-prone peptides
Truc Lam Pham1,2, Jennifer Zilke1,2, Christine Charlotte Müller3
1Institute of Organic Chemistry, Heidelberg University, Im Neuenheimer Feld 270 69120 Heidelberg Germany franziska.thomas@oci.uni-heidelberg.de.
Abstract:
This report describes the application of cyanosulfurylide (CSY)-protected aspartatic acid building blocks in microwave-assisted synthesis of aggregation-prone protein domains. We present a synthesis of Fmoc-Asp(CSY)-OH on a multigram scale, as well as procedures for the microwave-assisted synthesis of CSY-protected peptides, and CSY cleavage in partially folded or aggregation-prone peptides.
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